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JoVE Journal Developmental Biology
Extra Cellular Matrix-Based und Extra Cellular Matrix-Free Generation of Murine Hodenorganoide
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Extra Cellular Matrix-Based und Extra Cellular Matrix-Free Generation of Murine Hodenorganoide

Article DOI: 10.3791/61403-v • 09:18 min • October 7th, 2020
October 7th, 2020 •
1, 1, 1
1Department of Obstetrics and Gynecology, Feinberg School of Medicine, Northwestern University

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Hier werden vier Methoden zur Erzeugung von Hodenorganoiden aus primären neonatalen murinen Hodenzellzellen beschrieben, d.h. extrazelluläre Matrix (ECM) und ECM-freie 2D- und 3D-Kulturumgebungen. Diese Techniken haben mehrere Forschungsanwendungen und sind besonders nützlich für das Studium der Hodenentwicklung und Physiologie in vitro.

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Entwicklungsbiologie Ausgabe 164 Hoden Hodenorganoid extrazelluläre Matrix 2D 3D Baugruppe Tubuli endokrine Funktion
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