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Developmental Biology

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JoVE Journal Developmental Biology
Generazione extra di organoidi testicolari murini basati su matrice cellulare ed extra cellulare
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Generazione extra di organoidi testicolari murini basati su matrice cellulare ed extra cellulare

Article DOI: 10.3791/61403-v • 09:18 min • October 7th, 2020
October 7th, 2020 •
1, 1, 1
1Department of Obstetrics and Gynecology, Feinberg School of Medicine, Northwestern University

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Qui vengono descritti quattro metodi per generare organoidi testicolari da cellule testicolari murine neonatale primarie, cioè matrice extracellulare (ECM) e ambienti di coltura 2D e 3D senza ECM. Queste tecniche hanno molteplici applicazioni di ricerca e sono particolarmente utili per studiare lo sviluppo testicolare e la fisiologia in vitro.

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Biologia dello sviluppo Numero 164 testicolo organoide testicolare matrice extracellulare 2D 3D assemblaggio tubulo funzione endocrina
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