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JoVE Journal Developmental Biology
Geração extra-baseada em matriz celular e extra-celular livre de organoides testiculares de murina
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Geração extra-baseada em matriz celular e extra-celular livre de organoides testiculares de murina

Article DOI: 10.3791/61403-v • 09:18 min • October 7th, 2020
October 7th, 2020 •
1, 1, 1
1Department of Obstetrics and Gynecology, Feinberg School of Medicine, Northwestern University

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Aqui, são descritos quatro métodos para a geração de organoides testiculares testiculares de células testiculares neonatais primárias, ou seja, matriz extracelular (ECM) e ambientes de cultura 2D e 3D livres de ECM. Essas técnicas possuem múltiplas aplicações de pesquisa e são especialmente úteis para estudar desenvolvimento testicular e fisiologia in vitro.

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Biologia do Desenvolvimento Edição 164 testis organoide testicular matriz extracelular 2D 3D montagem túbulo função endócrina
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