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MCF7细胞单层中细胞基质粘附面积和细胞形状分布的定量
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Quantification of Cell-Substrate Adhesion Area and Cell Shape Distributions in MCF7 Cell Monolayers
Please note that all translations are automatically generated.
Click here for the English version.
MCF7细胞单层中细胞基质粘附面积和细胞形状分布的定量
DOI:
10.3791/61461-v
•
06:46 min
•
June 24, 2020
•
Maciej Wakula
,
Anna Balcerak
,
Urszula Smietanka
,
Mateusz Chmielarczyk
,
Ryszard Konopiński
,
Ewa A. Grzybowska
1
Maria Sklodowska-Curie National Research Institute of Oncology
Chapters
00:04
Introduction
00:33
Confluent Monolayer Generation
01:06
Focal Adhesion Analysis
03:13
Manual Cell Shape Analysis
04:04
Automated Cell Shape Analysis
04:54
Results: Representative Focal Adhesion and Cell Shape Distribution Analyses
06:18
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
本文描述了1)焦点粘附的大小和数量,2)细胞形状指数及其分布从MCF7细胞的汇合单层共聚焦图像。
Tags
Cell-substrate Adhesion
Cell Shape
MCF7 Cell Monolayers
Quantification
Confocal Microscopy
ImageJ
Focal Adhesion Analysis
Background Subtraction
Binary Conversion
Particle Analysis
ImageJ Macro
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