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MCF7细胞单层中细胞基质粘附面积和细胞形状分布的定量
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JoVE Journal Biology
Quantification of Cell-Substrate Adhesion Area and Cell Shape Distributions in MCF7 Cell Monolayers
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MCF7细胞单层中细胞基质粘附面积和细胞形状分布的定量

DOI:
10.3791/61461-v

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06:46 min

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June 24, 2020

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, , , , ,
1Maria Sklodowska-Curie National Research Institute of Oncology

Chapters

  • 00:04Introduction
  • 00:33Confluent Monolayer Generation
  • 01:06Focal Adhesion Analysis
  • 03:13Manual Cell Shape Analysis
  • 04:04Automated Cell Shape Analysis
  • 04:54Results: Representative Focal Adhesion and Cell Shape Distribution Analyses
  • 06:18Conclusion

Summary

Automatic Translation

Automatic Translation

本文描述了1）焦点粘附的大小和数量，2）细胞形状指数及其分布从MCF7细胞的汇合单层共聚焦图像。

Tags

Cell-substrate AdhesionCell ShapeMCF7 Cell MonolayersQuantificationConfocal MicroscopyImageJFocal Adhesion AnalysisBackground SubtractionBinary ConversionParticle AnalysisImageJ Macro

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