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Kvantificering af celleunderlagsadhæsionsområde og celleformfordelinger i MCF7-cellemonolag
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JoVE Journal Biology
Quantification of Cell-Substrate Adhesion Area and Cell Shape Distributions in MCF7 Cell Monolayers

Kvantificering af celleunderlagsadhæsionsområde og celleformfordelinger i MCF7-cellemonolag

DOI:
10.3791/61461-v

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06:46 min

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June 24, 2020

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, , , , ,
1Maria Sklodowska-Curie National Research Institute of Oncology

Chapters

  • 00:04Introduction
  • 00:33Confluent Monolayer Generation
  • 01:06Focal Adhesion Analysis
  • 03:13Manual Cell Shape Analysis
  • 04:04Automated Cell Shape Analysis
  • 04:54Results: Representative Focal Adhesion and Cell Shape Distribution Analyses
  • 06:18Conclusion

Summary

Automatic Translation

Automatic Translation

Artiklen beskriver kvantificering af 1) størrelsen og antallet af fokale sammenvoksninger og 2) celleform indeks og dens fordeling fra konfokale billeder af sammenløbet monolayers af MCF7 celler.

Tags

Cell-substrate AdhesionCell ShapeMCF7 Cell MonolayersQuantificationConfocal MicroscopyImageJFocal Adhesion AnalysisBackground SubtractionBinary ConversionParticle AnalysisImageJ Macro

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