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RNA Blot Analysis for the Detection and Quantification of Plant MicroRNAs
JoVE Journal
Biochemistry
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JoVE Journal Biochemistry
RNA Blot Analysis for the Detection and Quantification of Plant MicroRNAs
DOI:

14:41 min

July 11, 2020

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Chapters

  • 00:00Introduction
  • 01:27Step 1: Preparation of a 15% Denaturing Polyacrylamide Gel
  • 02:03Step 2: Assembly of the Glass Plates and Electrophresis Unit
  • 04:13Step 3: Preparation of Loading Dye and Samples
  • 05:38Step 4: Gel Electrophresis
  • 06:33Step 5: Preparation for Electro-blotting
  • 10:15Step 6: Preparation of Radiolabeled Probe
  • 10:50Step 7: Hybridization of the Blot
  • 12:36Representative Results
  • 13:24Conclusion

Summary

Automatic Translation

This method demonstrates use of the northern hybridization technique to detect miRNAs from total RNA extract.

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