Chapters
Summary
This protocol describes an application of single-molecule fluorescence in situ hybridization (smFISH) to measure the in vivo kinetics of mRNA synthesis and degradation.
Tags
MRNA Kinetics Escherichia Coli Two-color Single-molecule Fluorescence In Situ Hybridization (smFISH) Transcription MRNA Degradation Time Course Experiments Cover Slips Glass Slides Coplin Jar Ethanol Water Bath Ultrasonic Cater Sonication Hydrophobic Marker Poly L Lysine Time Course Experiment E.coli Culture
PLAYLIST
Biology
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Research • BiologyQuantifying Spatiotemporal Parameters of Cellular Exocytosis in Micropatterned Cells -
Research • BioengineeringMetabolic Profiling to Determine Bactericidal or Bacteriostatic Effects of New Natural Products using Isothermal Microcalorimetry -
Research • BiologyProbing mRNA Kinetics in Space and Time in Escherichia coli using Two-Color Single-Molecule Fluorescence In Situ Hybridization -
Research • BiologyPlanarian Scrunching as a Quantitative Behavioral Readout for Noxious Stimuli Sensing -
Research • BiologyQuantification of Cell-Substrate Adhesion Area and Cell Shape Distributions in MCF7 Cell Monolayers
Chemistry
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Research • ChemistryExcitonic Hamiltonians for Calculating Optical Absorption Spectra and Optoelectronic Properties of Molecular Aggregates and Solids -
Research • ChemistryAutomated Sample Multiplexing by using Combined Precursor Isotopic Labeling and Isobaric Tagging (cPILOT) -
Research • ChemistryHyperspectral Imaging as a Tool to Study Optical Anisotropy in Lanthanide-Based Molecular Single Crystals -
Research • ChemistryMass Spectrometry-Guided Genome Mining as a Tool to Uncover Novel Natural Products -
Research • ChemistryA Microwave-Assisted Direct Heteroarylation of Ketones Using Transition Metal Catalysis
Engineering
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Research • EngineeringTerahertz Imaging and Characterization Protocol for Freshly Excised Breast Cancer Tumors -
Research • EngineeringA Random-displacement Measurement by Combining a Magnetic Scale and Two Fiber Bragg Gratings -
Research • EngineeringFabricating van der Waals Heterostructures with Precise Rotational Alignment -
Research • EngineeringCutting Procedures, Tensile Testing, and Ageing of Flexible Unidirectional Composite Laminates -
Research • EngineeringDeveloping High Performance GaP/Si Heterojunction Solar Cells
Clinical Science
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Research • MedicineLung CT Segmentation to Identify Consolidations and Ground Glass Areas for Quantitative Assesment of SARS-CoV Pneumonia -
Research • MedicineEvaluation of Capnography Sampling Line Compatibility and Accuracy when Used with a Portable Capnography Monitor -
Research • MedicineAssessment of Dependence in Activities of Daily Living Among Older Patients in an Acute Care Unit -
Research • MedicineInduction of Accelerated Atherosclerosis in Mice: The "Wire-Injury" Model -
Research • MedicineAn In Vitro Hemodynamic Loop Model to Investigate the Hemocytocompatibility and Host Cell Activation of Vascular Medical Devices
