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February 01, 2010
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Summary
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MYTH allows the sensitive detection of transient and stable interactions between proteins that are expressed in the model organism Saccharomyces cerevisiae. It has been successfully applied to study exogenous and yeast integral membrane proteins in order to identify their interacting partners in a high throughput manner.
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Cite this Article
Snider, J., Kittanakom, S., Curak, J., Stagljar, I. Split-Ubiquitin Based Membrane Yeast Two-Hybrid (MYTH) System: A Powerful Tool For Identifying Protein-Protein Interactions. J. Vis. Exp. (36), e1698, doi:10.3791/1698 (2010).
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