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Biology

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Split-Ubiquitin Based Membrane Yeast Two-Hybrid (MYTH) System
 

Split-Ubiquitin Based Membrane Yeast Two-Hybrid (MYTH) System: A Powerful Tool For Identifying Protein-Protein Interactions

Article doi: 10.3791/1698
February 1st, 2010

Summary February 1st, 2010

MYTH allows the sensitive detection of transient and stable interactions between proteins that are expressed in the model organism Saccharomyces cerevisiae. It has been successfully applied to study exogenous and yeast integral membrane proteins in order to identify their interacting partners in a high throughput manner.

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