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Journal / Biology / Sondering mRNA kinetik i rum og tid i Escherichia …
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Biology

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Sondering mRNA kinetik i rum og tid i Escherichia coli ved hjælp af to-color single-molecule fluorescens In Situ Hybridization

Article DOI: 10.3791/61520-v • 10:01 min • July 30th, 2020
July 30th, 2020 •
1,2,3,4, 1,2
1Department of Physics, University of Illinois at Urbana-Champaign, 2Center for the Physics of Living Cells, University of Illinois at Urbana-Champaign, 3Carl R. Woese Institute for Genomic Biology, University of Illinois at Urbana-Champaign, 4Center for Biophysics and Quantitative Biology, University of Illinois at Urbana-Champaign

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Denne protokol beskriver en anvendelse af enkelt-molekyle fluorescens in situ hybridisering (smFISH) til at måle in vivo kinetik af mRNA syntese og nedbrydning.

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Biologi smFISH RNA imaging mRNA lokalisering enkelt celle enkelt molekyle transskription transskription forlængelse for tidlig opsigelse mRNA nedbrydning
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