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Journal / Biology / Fluorescens in situ hybridisering på DNA halo…
JoVE Journal
Biology

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JoVE Journal Biology
Fluorescens in situ hybridisering på DNA halo præparater til at afsløre hele kromosomer, telomerer og gen loci
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Fluorescens in situ hybridisering på DNA halo præparater til at afsløre hele kromosomer, telomerer og gen loci

Article DOI: 10.3791/62017-v • 09:07 min • March 4th, 2021
March 4th, 2021 •
1, 2, 1, 2
1Laboratory of Nuclear and Genomic Health, Centre for Genome Engineering and Maintenance, Division of Biosciences, Department of Life Sciences, College of Health, Medicine and Life Sciences, Brunel University London, 2Biosciences, Department of Clinical, Pharmaceutical and Biological Science, School of Life and Medical Sciences, University of Hertfordshire

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Kombination af DNA-halopræparater med fluorescens in situ-hybridisering muliggør højopløsningsanalyse af genomiske interaktioner med nukleoskelettet. Vedhæftet genom fører til hybridiserede fluorescerende signaler placeret i de resterende ekstraherede kerner, mens ikke-bundet genom er i DNA-haloen, der omgiver de resterende kerner.

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Denne måned i JoVE udgave 169 DNA-haloforberedelse nuklear matrix nukleoskelet genomorganisation DNA-sløjfer kromosomområder udvidede kromatinfibre matrixvedhæftningsregioner telomerer
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