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JoVE Journal
Biology

This content is Open Access.

用于大规模表观遗传学研究的半自动ChIP-Seq程序
 
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用于大规模表观遗传学研究的半自动ChIP-Seq程序

Article DOI: 10.3791/61617-v 08:04 min August 13th, 2020
August 13th, 2020

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Summary

Please note that all translations are automatically generated.

Click here for the English version.

本文描述了使用ChIP液体处理平台对与特定组蛋白修饰(H3K27ac)相关的DNA区域的半自动,微量尺度的ChIP-Seq方案,然后使用标记进行文库制备。该程序包括出于质量和数量目的的对照评估,并且可以用于其他组蛋白修饰或转录因子。

Tags

生物学,第162期,ChIP-Seq,ChIP液体处理器,标记,H3K27ac,低输入,切屑,自动化
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