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En model membran platform for rekonstituerende Mitokondrielle Membran Dynamics
JoVE Journal
Biochemistry
This content is Free Access.
JoVE Journal Biochemistry
A Model Membrane Platform for Reconstituting Mitochondrial Membrane Dynamics
DOI:

10:31 min

September 02, 2020

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Chapters

  • 00:04Introduction
  • 00:37Fabrication of Lipid Bilayers
  • 04:41Protein Reconstitution into the Polymer-tethered Lipid Bilayer
  • 06:02Preparation of Proteoliposomes
  • 07:46Results: Reconstituted Transmembrane Protein Diffusion and Detection of Membrane Tethering, Lipid Hemifusion, and Pore Opening
  • 09:42Conclusion

Summary

Automatic Translation

Mitokondriel fusion er en vigtig hjemostatisk reaktion underliggende mitokondrielle dynamik. Beskrevet her er en in vitro rekonstitution system til at studere mitokondrielle indre membran fusion, der kan løse membran tøjring, docking, hemifusion, og pore åbning. Alsidigheden i denne tilgang til at udforske cellemembransystemer diskuteres.

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