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Journal / Biochemistry / En modell membran plattform för rekonstituering…
JoVE Journal
Biochemistry

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JoVE Journal Biochemistry
En modell membran plattform för rekonstituering mitokondriellt membran Dynamics
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En modell membran plattform för rekonstituering mitokondriellt membran Dynamics

Article DOI: 10.3791/61620-v • 10:31 min • September 2nd, 2020
September 2nd, 2020 •
1, 1, 2, 1,3
1Department of Molecular Biology, Massachusetts General Hospital, 2Department of Chemistry, University of Akron, 3Department of Genetics, Harvard Medical School

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Mitokondriell fusion är en viktig homeostatisk reaktion underliggande mitokondriell dynamik. Beskrivs här är en in vitro reconstitution system för att studera mitokondriellt inre-membran fusion som kan lösa membran tjudra, dockning, hemifusion och poröppning. Mångsidigheten hos detta tillvägagångssätt i utforska cellmembran system diskuteras.

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Denna månad i JoVE mitokondriellt inre membran Opa1 polymer-bundna lipid bilayer in vitro rekonstituering membran fusion TIRF mikroskopi
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