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JoVE Journal Neuroscience
Afleiding, expansie, cryopreservatie en karakterisering van hersenmicrovasculaire endotheelcellen uit door de mens geïnduceerde pluripotente stamcellen
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Afleiding, expansie, cryopreservatie en karakterisering van hersenmicrovasculaire endotheelcellen uit door de mens geïnduceerde pluripotente stamcellen

Article DOI: 10.3791/61629-v • 08:04 min • November 19th, 2020
November 19th, 2020 •
1,2,3, 1,2,3,4, 1,3,4,5
1Center for Genomic Medicine, Massachusetts General Hospital, 2Department of Psychiatry, Beth Israel Deaconess Medical Center, 3Chemical Biology and Therapeutic Science Program, Broad Institute of MIT and Harvard, 4Department of Psychiatry, Harvard Medical School, 5Schizophrenia and Bipolar Disorder Program, McLean Hospital

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Dit protocol beschrijft een aangepaste methode om microvasculaire endotheelcellen van de hersenen af te leiden, uit te breiden en cryopreserveren, verkregen door het differentiëren van door de mens geïnduceerde pluripotente stamcellen, en om de eigenschappen van de bloed-hersenbarrière in een ex vivo model te bestuderen.

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Neurowetenschappen Human induced pluripotent stam cells brain microvascular endothelial cells blood brain barrier trans-endothelial electrical resistance transporter activity microvascular schizophrenia bipolaire disorder passaging expansion cryopreservation
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