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Neuroscience
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ヒト人工多能性幹細胞由来脳微小血管内皮細胞の誘導、拡大、凍結保存および特性評価
JoVE Journal
Neuroscience
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JoVE Journal
Neuroscience
Derivation, Expansion, Cryopreservation and Characterization of Brain Microvascular Endothelial Cells from Human Induced Pluripotent Stem Cells
Please note that all translations are automatically generated.
Click here for the English version.
ヒト人工多能性幹細胞由来脳微小血管内皮細胞の誘導、拡大、凍結保存および特性評価
DOI:
10.3791/61629-v
•
08:04 min
•
November 19, 2020
•
Sovannarath Pong
2,3
,
Paulo Lizano
2,3,4
,
Rakesh Karmacharya
3,4,5
1
Center for Genomic Medicine
,
Massachusetts General Hospital
,
2
Department of Psychiatry
,
Beth Israel Deaconess Medical Center
,
3
Chemical Biology and Therapeutic Science Program
,
Broad Institute of MIT and Harvard
,
4
Department of Psychiatry
,
Harvard Medical School
,
5
Schizophrenia and Bipolar Disorder Program
,
McLean Hospital
Chapters
00:04
Introduction
00:38
Brain Microvascular Endothelial Cell (BMEC) Differentiation from Human Induced Pluripotent Stem Cell (iPSC)
01:46
iPSC-Derived BMEC Subculture and Purification
03:02
Trans-Endothelial Electrical Resistance (TEER) Measurement and Analysis
04:09
Efflux Transporter Activity and Analysis
05:22
Results: Representative BMEC Differentiation and Characterization After Passaging, Subculture, and Cryopreservation
07:18
Conclusion
Summary
Automatic Translation
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Automatic Translation
本プロトコルは、ヒト人工多能性幹細胞を分化して得られる脳内血管内皮細胞を導き出し、拡大、および凍結保存し、
また、ex vivo
モデルにおける血液脳関門特性を研究するための適応法を詳述する。
Tags
Brain Microvascular Endothelial Cells
Human Induced Pluripotent Stem Cells
Blood-brain Barrier
BMEC Derivation
BMEC Expansion
BMEC Cryopreservation
BMEC Characterization
TEER Analysis
Efflux Transporter Activity
Disease Modeling
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