Journal
/
/
ヒト人工多能性幹細胞由来脳微小血管内皮細胞の誘導、拡大、凍結保存および特性評価
JoVE Journal
Neuroscience
A subscription to JoVE is required to view this content.  Sign in or start your free trial.
JoVE Journal Neuroscience
Derivation, Expansion, Cryopreservation and Characterization of Brain Microvascular Endothelial Cells from Human Induced Pluripotent Stem Cells
DOI:

08:04 min

November 19, 2020

, ,

Chapters

  • 00:04Introduction
  • 00:38Brain Microvascular Endothelial Cell (BMEC) Differentiation from Human Induced Pluripotent Stem Cell (iPSC)
  • 01:46iPSC-Derived BMEC Subculture and Purification
  • 03:02Trans-Endothelial Electrical Resistance (TEER) Measurement and Analysis
  • 04:09Efflux Transporter Activity and Analysis
  • 05:22Results: Representative BMEC Differentiation and Characterization After Passaging, Subculture, and Cryopreservation
  • 07:18Conclusion

Summary

Automatic Translation

本プロトコルは、ヒト人工多能性幹細胞を分化して得られる脳内血管内皮細胞を導き出し、拡大、および凍結保存し、 また、ex vivo モデルにおける血液脳関門特性を研究するための適応法を詳述する。

Related Videos

Read Article