/
/
Derivazione, espansione, crioconservazione e caratterizzazione di cellule endoteliali microvascolari cerebrali da cellule staminali pluripotenti indotte dall'uomo
A subscription to JoVE is required to view this content. Sign in or start your free trial.
1Center for Genomic Medicine,Massachusetts General Hospital, 2Department of Psychiatry,Beth Israel Deaconess Medical Center, 3Chemical Biology and Therapeutic Science Program,Broad Institute of MIT and Harvard, 4Department of Psychiatry,Harvard Medical School, 5Schizophrenia and Bipolar Disorder Program,McLean Hospital
Chapters
- 00:04Introduction
- 00:38Brain Microvascular Endothelial Cell (BMEC) Differentiation from Human Induced Pluripotent Stem Cell (iPSC)
- 01:46iPSC-Derived BMEC Subculture and Purification
- 03:02Trans-Endothelial Electrical Resistance (TEER) Measurement and Analysis
- 04:09Efflux Transporter Activity and Analysis
- 05:22Results: Representative BMEC Differentiation and Characterization After Passaging, Subculture, and Cryopreservation
- 07:18Conclusion
Questo protocollo descrive in dettaglio un metodo adattato per derivare, espandere e crioconservare le cellule endoteliali microvascolari cerebrali ottenute differenziando le cellule staminali pluripotenti indotte dall'uomo e per studiare le proprietà della barriera eculare nel sangue in un modello ex vivo.
