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Derivazione, espansione, crioconservazione e caratterizzazione di cellule endoteliali microvascolari cerebrali da cellule staminali pluripotenti indotte dall'uomo
JoVE Journal
Neuroscience
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JoVE Journal Neuroscience
Derivation, Expansion, Cryopreservation and Characterization of Brain Microvascular Endothelial Cells from Human Induced Pluripotent Stem Cells
DOI:

08:04 min

November 19, 2020

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Chapters

  • 00:04Introduction
  • 00:38Brain Microvascular Endothelial Cell (BMEC) Differentiation from Human Induced Pluripotent Stem Cell (iPSC)
  • 01:46iPSC-Derived BMEC Subculture and Purification
  • 03:02Trans-Endothelial Electrical Resistance (TEER) Measurement and Analysis
  • 04:09Efflux Transporter Activity and Analysis
  • 05:22Results: Representative BMEC Differentiation and Characterization After Passaging, Subculture, and Cryopreservation
  • 07:18Conclusion

Summary

Automatic Translation

Questo protocollo descrive in dettaglio un metodo adattato per derivare, espandere e crioconservare le cellule endoteliali microvascolari cerebrali ottenute differenziando le cellule staminali pluripotenti indotte dall'uomo e per studiare le proprietà della barriera eculare nel sangue in un modello ex vivo.

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