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Journal / Biochemistry / Transkripsiyonel Aktif Alellerin Tek Molekül FISH …
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Biochemistry

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JoVE Journal Biochemistry
Transkripsiyonel Aktif Alellerin Tek Molekül FISH ile Tek Hücre Analizi
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Transkripsiyonel Aktif Alellerin Tek Molekül FISH ile Tek Hücre Analizi

Article DOI: 10.3791/61680-v • 06:26 min • September 20th, 2020
September 20th, 2020 •
1,2, 1,3, 1,2, 1,2, 1,2,3,4
1GCC Center for Advanced Microscopy and Image Informatics, 2Department of Molecular and Cellular Biology, Baylor College of Medicine, 3Center for Translational Cancer Research, Institute of Biosciences and Technology, Texas A&M University, 4Department of Pharmacology and Chemical Biology, Baylor College of Medicine

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Tek moleküllü RNA floresan in situ hibridizasyon (smFISH), belirli RNA'ların seviyelerini ve lokalizasyonunu tek hücre düzeyinde doğru bir şekilde ölçmek için bir yöntemdir. Burada, belirli RNA'ların tek hücreli nicelleştirilmesi için ıslak tezgah işleme, görüntüleme ve görüntü analizi için onaylanmış laboratuvar protokollerimizi rapor ediyoruz.

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Biyokimya Sayı 163 Tek molekül fish görüntüleme görüntü analizi gen transkripsiyon
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