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Journal / Biochemistry / Einzelzellanalyse transkriptionell aktiver Allele …
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Biochemistry

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JoVE Journal Biochemistry
Einzelzellanalyse transkriptionell aktiver Allele mittels Einzelmolekül FISH
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Lipid Index Determination by Liquid Fluorescence Recovery in the Fungal Pathogen Ustilago Maydis
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Einzelzellanalyse transkriptionell aktiver Allele mittels Einzelmolekül FISH

Article DOI: 10.3791/61680-v • 06:26 min • September 20th, 2020
September 20th, 2020 •
1,2, 1,3, 1,2, 1,2, 1,2,3,4
1GCC Center for Advanced Microscopy and Image Informatics, 2Department of Molecular and Cellular Biology, Baylor College of Medicine, 3Center for Translational Cancer Research, Institute of Biosciences and Technology, Texas A&M University, 4Department of Pharmacology and Chemical Biology, Baylor College of Medicine

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Die Einzelmolekül-RNA-Fluoreszenz-in-situ-Hybridisierung (smFISH) ist eine Methode zur genauen Quantifizierung der Konzentrationen und Lokalisierung spezifischer RNAs auf Einzelzellebene. Hier berichten wir über unsere validierten Laborprotokolle für die Nasstischverarbeitung, Bildgebung und Bildanalyse zur Einzelzellquantifizierung spezifischer RNAs.

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Biochemie Heft 163 Einzelmolekül FISH Bildgebung Bildanalyse Gentranskription
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