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从高梁叶组织中分离出希石,用于自上而下质量光谱分析潜在的表观遗传标记
JoVE Journal
Biochemistry
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JoVE Journal Biochemistry
Isolation of Histone from Sorghum Leaf Tissue for Top Down Mass Spectrometry Profiling of Potential Epigenetic Markers
DOI:

07:10 min

March 04, 2021

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Chapters

  • 00:05Introduction
  • 00:40Nuclei Isolation from Sorghum Leaf Sample
  • 02:13Isolation of Nuclei from Remaining Cytoplasmic Contaminants
  • 03:05Nuclei Lysis and Histone Extraction
  • 04:39Results: Qualitative & Quantitative Analysis of the Identified Histones
  • 06:36Conclusion

Summary

Automatic Translation

该方案的制定是为了有效地从高梁叶材料中提取完整的组织结石,用于分析结石转化后的修饰,这些修饰可以作为潜在的表观遗传标记,以帮助工程抗旱作物。

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