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组织样品质谱基猎枪蛋白造影学的综合工作流程
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Biology
Comprehensive Workflow of Mass Spectrometry-based Shotgun Proteomics of Tissue Samples
Please note that all translations are automatically generated.
Click here for the English version.
组织样品质谱基猎枪蛋白造影学的综合工作流程
DOI:
10.3791/61786-v
•
14:51 min
•
November 13, 2021
•
Ayushi Verma*
1
,
Vipin Kumar*
1
,
Saicharan Ghantasala
,
Shuvolina Mukherjee
,
Sanjeeva Srivastava
1
Department of Biosciences and Bioengineering
,
Indian Institute of Technology Bombay
Chapters
00:00
Introduction
00:42
Tissue Lysate Preparation
01:51
Quantification and Quality Check of Tissue Lysates
02:33
Enzymatic Digestion of Proteins
03:57
Desalting of Digested Peptides
05:16
Quantification of Desalted Peptides
05:58
Labeling of Digested Peptides using iTRAQ Reagents
08:14
Liquid Chromatography Set-up
09:25
Mass Spectrometry Set-up
12:30
Representative Results
13:45
Conclusions
Summary
Automatic Translation
English (Original)
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Automatic Translation
所述协议使用两种方法对组织样本进行优化定量蛋白学分析:基于标签和无标签量化。基于标签的方法具有更精确的蛋白质量化优势,而无标签方法更具成本效益,用于分析数百个组位样本。
Tags
Mass Spectrometry
Shotgun Proteomics
Quantitative Proteomics
Biomarker Discovery
Label-free
Label-based
Probe Sonicator
Bead Beater
Bradford Assay
SDS-PAGE
In-solution Digestion
TCEP
Iodoacetamide
Trypsin Digestion
C18 Stage Tips
Desalting
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