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Identification of EGFR and RAS Inhibitors using Caenorhabditis elegans
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Cancer Research
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JoVE Journal Cancer Research
Identification of EGFR and RAS Inhibitors using Caenorhabditis elegans

Identification of EGFR and RAS Inhibitors using Caenorhabditis elegans

DOI:
10.3791/61788-v

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08:12 min

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October 05, 2020

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, , , , ,
1Department of Diagnostic and Biomedical Sciences, School of Dentistry,University of Texas Health Science Center, 2Department of Integrative Biology and Pharmacology, McGovern Medical School,University of Texas Health Science Center

Chapters

  • 00:05Introduction
  • 00:26Preparation of a Synchronous C. elegans Culture
  • 02:51Preparation of Drug Assays
  • 04:30Observation of the Muv Phenotype in the let-60, let-23 and lin-1 Strains
  • 06:38Results: R-fendiline Alters let-60 and let-23 Function in C. elegans in a Dose-dependent Manner
  • 07:25Conclusion

Summary

Automatic Translation

Automatic Translation

The genetically tractable nematode Caenorhabditis elegans can be used as a simple and inexpensive model for drug discovery. Described here is a protocol to identify anticancer therapeutics that inhibit the downstream signaling of RAS and EGFR proteins.

Tags

EGFRRASCaenorhabditis ElegansDrug DiscoveryAnti-EGFRAnti-RASTherapeuticsE. Coli OP50NGM PlatesGravid Adult WormsWorm LysisEmbryosL1 LarvaeM9W

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