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Identification of OTX1 and OTX2 As Two Possible Molecular Markers for Sinonasal Carcinomas and Olfactory Neuroblastomas
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Cancer Research
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JoVE Journal Cancer Research
Identification of OTX1 and OTX2 As Two Possible Molecular Markers for Sinonasal Carcinomas and Olfactory Neuroblastomas

Identification of OTX1 and OTX2 As Two Possible Molecular Markers for Sinonasal Carcinomas and Olfactory Neuroblastomas

DOI:
10.3791/56880-v

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07:00 min

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February 28, 2019

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, , , , , , , , , , , , , ,
1Department of Medicine and Surgery,University of Insubria, 2Department of Surgical and Morphological Science,University of Insubria, 3Department of Biotechnology and Life Science,University of Insubria

Chapters

  • 00:04Title
  • 00:58Immunochemistry
  • 03:03Real-time PCR and Data Analysis
  • 04:56Results: Representative Images of OTX Immunohistochemical Expression in Control and Neoplastic Tissues
  • 05:47Conclusion

Summary

Automatic Translation

Automatic Translation

Homeobox genes are regulatory genes often associated with tumors in the adult organisms. We investigated their comparative expression by immunohistochemical and real-time PCR analysis, in normal and inflammatory nasal mucosae and in sinonasal neoplasms in order to use them as possible diagnostic and therapeutic targets.

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Keywords: OTX1OTX2Sinonasal CarcinomasOlfactory NeuroblastomasMolecular MarkersRNA ExtractionReal-time PCRImmunohistochemistryFFPE SamplesAntigen RetrievalTriton XSecondary AntibodyABC PeroxidaseDiaminobenzeneHematoxylinDehydrationProbe-based PCR

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