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Developmental Biology

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JoVE Journal Developmental Biology
Transillumination-assistert spredning av spesifikke stadier av musen seminiferøs epitelsyklus for nedstrøms immunstaining analyser
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Transillumination-assistert spredning av spesifikke stadier av musen seminiferøs epitelsyklus for nedstrøms immunstaining analyser

Article DOI: 10.3791/61800-v • 09:59 min • October 7th, 2020
October 7th, 2020 •
1, 1, 1, 1, 3,4, 1,2, 3,4, 1, 1
1Institute of Biomedicine, Integrative Physiology and Pharmacology Unit, University of Turku, Turku, Finland, 2Department of Pediatrics, Turku University Hospital, Turku, Finland, 3Centre for Reproductive Health, Hudson Institute of Medical Research, Melbourne, VIC 3168, Australia, 4Australian Regenerative Medicine Institute, Monash University, Melbourne, VIC 3800, Australia

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Denne protokollen beskriver transillumination-assistert mikrodisseksjon av segmenter av voksen mus seminiferøse tubuli som representerer bestemte stadier av seminiferøs epitelsyklus, og celletyper deri, og påfølgende immunstaining av squash preparater og intakt tubule segmenter.

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Utviklingsbiologi Utgave 164 mus spermatogenese seminiferøs epitel scene transillumination squash forberedelse full-mount farging spermiogenese
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