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Journal / Developmental Biology / CRISPR/Cas9-mediert svært effektiv genmålretting…
JoVE Journal
Developmental Biology

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JoVE Journal Developmental Biology
CRISPR/Cas9-mediert svært effektiv genmålretting i embryonale stamceller for utvikling av genmanipulerte musemodeller
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CRISPR/Cas9-mediert svært effektiv genmålretting i embryonale stamceller for utvikling av genmanipulerte musemodeller

Article DOI: 10.3791/64385-v • 10:57 min • August 24th, 2022
August 24th, 2022 •
*1, *2, 1,2
1Laboratory of Reproductive Systems Biology, Center for Experimental Medicine and Systems Biology, The Institute of Medical Science, The University of Tokyo, 2Research Institute for Microbial Diseases, Osaka University
* These authors contributed equally

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Her presenterer vi en protokoll for utvikling av genmodifiserte musemodeller ved bruk av embryonale stamceller, spesielt for store DNA-knock-in (KI). Denne protokollen er innstilt ved hjelp av CRISPR / Cas9 genomredigering, noe som resulterer i betydelig forbedret KI-effektivitet sammenlignet med den konvensjonelle homologe rekombinasjonsmedierte lineariserte DNA-målrettingsmetoden.

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Utviklingsbiologi utgave 186
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