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A Simple Dry Sectioning Method for Obtaining Whole-Seed-Sized Resin Section and Its Applications
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A Simple Dry Sectioning Method for Obtaining Whole-Seed-Sized Resin Section and Its Applications

A Simple Dry Sectioning Method for Obtaining Whole-Seed-Sized Resin Section and Its Applications

DOI:
10.3791/61822-v

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10:10 min

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January 23, 2021

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, , , ,
1Key Laboratory of Crop Genetics and Physiology of Jiangsu Province/Jiangsu Key Laboratory of Crop Genomics and Molecular Breeding,Yangzhou University, 2Co-Innovation Center for Modern Production Technology of Grain Crops of Jiangsu Province/Joint International Research Laboratory of Agriculture & Agri-Product Safety of the Ministry of Education,Yangzhou University

Chapters

  • 00:00Introduction
  • 01:12Preparation of Resin-embedded Seed
  • 03:36Dry Sectioning for Preparation Whole-seed-sized Section
  • 05:16Staining and Observation of Section
  • 06:18Quantitative Analysis of Morphology Parameters
  • 06:37Results
  • 09:33Conclusion

Summary

Automatic Translation

Automatic Translation

This technique allows for the fast and simple preparation of whole-seed-sized resin section for the observation and analysis of cells, starch granules, and protein bodies in different regions of the seed.

Tags

Dry SectioningWhole-seed-sized Resin SectionLR White ResinCereal Mature KernelsCell MorphologyStarch GranulesProtein BodiesSample PreparationFixationDehydrationInfiltrationEmbeddingLight MicroscopyQuantitative Analysis

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