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JoVE Journal
Immunology and Infection

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一种基于DNA/Ki67的流式细胞术测定法,用于接种疫苗的小鼠抗原特异性CD8 T细胞的细胞周期分析
 
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一种基于DNA/Ki67的流式细胞术测定法,用于接种疫苗的小鼠抗原特异性CD8 T细胞的细胞周期分析

Article DOI: 10.3791/61867-v 09:17 min January 5th, 2021
January 5th, 2021

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Summary

Please note that all translations are automatically generated.

Click here for the English version.

克隆扩增是抗原特异性T细胞反应的关键特征。然而,抗原反应T细胞的细胞周期研究不佳,部分原因是技术限制。我们描述了一种流式细胞术方法,用于分析接种疫苗小鼠的脾脏和淋巴结中的克隆扩增抗原特异性CD8 T细胞。

Tags

免疫学和感染,第167期,抗原特异性CD8 T细胞,细胞周期,Ki67,DNA染料,流式细胞术,脾脏,淋巴结,小鼠
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