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Journal
/
Developmental Biology
/
晚期胚胎
果蝇
性腺的解剖和活体成像
JoVE Journal
Developmental Biology
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JoVE Journal
Developmental Biology
Dissection and Live-Imaging of the Late Embryonic
Drosophila
Gonad
Please note that all translations are automatically generated.
Click here for the English version.
晚期胚胎
果蝇
性腺的解剖和活体成像
DOI:
10.3791/61872-v
•
09:08 min
•
October 17, 2020
•
Kara A. Nelson
,
Bailey N. Warder
,
Stephen DiNardo
,
Lauren Anllo
1
Cell and Developmental Biology Department and the Penn Institute for Regenerative Medicine, Perelman School of Medicine
,
University of Pennsylvania
Chapters
00:05
Introduction
00:46
Day-of-Dissection Preparation
02:35
Dissection
04:53
Imaging
06:46
Results: The Live-Imaging Results of the Cultured Gonads, Their Health, and Niche Compaction
08:15
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
在这里,我们提供了对晚期胚胎
果蝇
雄性性腺进行现场成像所需的解剖方案。该协议将允许在正常条件下或在转基因或药理学操作之后观察动态细胞过程。
Tags
Live Imaging
Gonadogenesis
Dissection
Drosophila Embryo
Vitelline Membrane
Ringer’s Solution
Heptane Glue
Tungsten Needle
Poly-D-Lysine
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