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Pupal- og voksenindsprøjtninger til RNAi- og CRISPR-genredigering i Nasonia vitripennis
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Pupal- og voksenindsprøjtninger til RNAi- og CRISPR-genredigering i Nasonia vitripennis

Article DOI: 10.3791/61892-v • 08:41 min • December 4th, 2020
December 4th, 2020 •
*1, *1, 2, 1
1Division of Biological Sciences, Section of Cell and Developmental Biology, University of California, San Diego, 2Department of Entomology, The Center for Infectious Disease Dynamics, The Huck Institutes of the Life Sciences, The Pennsylvania State University
* These authors contributed equally

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Her beskriver vi metoder til effektive pupal- og voksenindsprøjtninger i Nasonia vitripennis som tilgængelige alternativer til embryomikroinjektion, der muliggør funktionel analyse af gener af interesse ved hjælp af enten RNA-lyddæmpning via RNA-interferens (RNAi) eller gen knockout via CRISPR / Cas9 genomredigering.

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Biologi Problem 166 Nasonia vitripennis CRISPR/Cas9 ReMOT BAPC-assisteret CRISPR Voksen injektioner RNAi
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