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JoVE Journal Biology
Puppen- und Erwachseneninjektionen zur RNAi- und CRISPR-Genbearbeitung bei Nasonia vitripennis
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Puppen- und Erwachseneninjektionen zur RNAi- und CRISPR-Genbearbeitung bei Nasonia vitripennis

Article DOI: 10.3791/61892-v
December 4th, 2020 •
*1, *1, 2, 1
1Division of Biological Sciences, Section of Cell and Developmental Biology, University of California, San Diego, 2Department of Entomology, The Center for Infectious Disease Dynamics, The Huck Institutes of the Life Sciences, The Pennsylvania State University
* These authors contributed equally

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December 4th, 2020

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Hier beschreiben wir Methoden für effiziente Puppen- und Erwachseneninjektionen in Nasonia vitripennis als zugängliche Alternativen zur Embryo-Mikroinjektion, die eine funktionelle Analyse von Genen von Interesse entweder mit RNA-Silencing über RNA-Interferenz (RNAi) oder Gen-Knockout durch CRISPR/Cas9-Genom-Editing ermöglichen.

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