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JoVE Journal Biology
나소니아 vitripennis에서 RNAi와 CRISPR 유전자 편집을위한 푸팔과 성인 주사
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나소니아 vitripennis에서 RNAi와 CRISPR 유전자 편집을위한 푸팔과 성인 주사

Article DOI: 10.3791/61892-v
December 4th, 2020 •
*1, *1, 2, 1
1Division of Biological Sciences, Section of Cell and Developmental Biology, University of California, San Diego, 2Department of Entomology, The Center for Infectious Disease Dynamics, The Huck Institutes of the Life Sciences, The Pennsylvania State University
* These authors contributed equally

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December 4th, 2020

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여기서, 우리는 NASOnia vitripennis에 있는 효율적인 pupal 및 성인 주사를 위한 방법을 태아 micro주입에 접근하는 대안으로, RNA 간섭 (RNAi) 또는 CRISPR/Cas9 게놈 편집을 통해 유전자 녹아웃을 통해 RNA 침묵을 사용하여 관심있는 유전자의 기능적 분석을 가능하게 하는 방법을 기술합니다.

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