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Inyecciones pupales y para adultos para RNAi y edición de genes CRISPR en Nasonia vitripennis
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Inyecciones pupales y para adultos para RNAi y edición de genes CRISPR en Nasonia vitripennis

Article DOI: 10.3791/61892-v
December 4th, 2020 •
*1, *1, 2, 1
1Division of Biological Sciences, Section of Cell and Developmental Biology, University of California, San Diego, 2Department of Entomology, The Center for Infectious Disease Dynamics, The Huck Institutes of the Life Sciences, The Pennsylvania State University
* These authors contributed equally

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December 4th, 2020

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Aquí, se describen los métodos para las inyecciones pupales y adultos eficientes en Nasonia vitripennis como alternativas accesibles a la microinyección embrionaria, lo que permite el análisis funcional de los genes de interés utilizando ya sea arn-silenciamiento a través de la interferencia de ARN (RNAi) o knockout gen a través de CRISPR / Cas9 edición del genoma.

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