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Iniezioni pupali e adulte per l'editing genico RNAi e CRISPR a Nasonia al vetripennis
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Iniezioni pupali e adulte per l'editing genico RNAi e CRISPR a Nasonia al vetripennis

Article DOI: 10.3791/61892-v • 08:41 min • December 4th, 2020
December 4th, 2020 •
*1, *1, 2, 1
1Division of Biological Sciences, Section of Cell and Developmental Biology, University of California, San Diego, 2Department of Entomology, The Center for Infectious Disease Dynamics, The Huck Institutes of the Life Sciences, The Pennsylvania State University
* These authors contributed equally

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Qui, descriviamo i metodi per iniezioni pupali e adulte efficienti in Nasonia vitripennis come alternative accessibili alla microiniezione embrionale, consentendo l'analisi funzionale dei geni di interesse utilizzando il silenziamento dell'RNA tramite interferenza dell'RNA (RNAi) o knockout genico tramite l'editing del genoma CRISPR / Cas9.

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Biologia Numero 166 Nasonia vetripennis,CRISPR/Cas9 ReMOT CRISPR assistito da BAPC Iniezioni per adulti RNAi
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