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Journal / Genetics / सैंड फ्लाई (Phlebotomus papatasi) CRISPR के लिए…
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Genetics

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JoVE Journal Genetics
सैंड फ्लाई (Phlebotomus papatasi) CRISPR के लिए भ्रूण माइक्रोइंजेक्शन/
Determining 3'-Termini and Sequences of Nascent Single-Stranded Viral DNA Molecules during HIV-1 Reverse Transcription in Infected Cells
Journal (Genetics)
Determining 3'-Termini and Sequences of Nascent Single-Stranded Viral DNA Molecules during HIV-1 Reverse Transcription in Infected Cells
RNA Next-Generation Sequencing and a Bioinformatics Pipeline to Identify Expressed LINE-1s at the Locus-Specific Level
Journal (Genetics)
RNA Next-Generation Sequencing and a Bioinformatics Pipeline to Identify Expressed LINE-1s at the Locus-Specific Level
 
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सैंड फ्लाई (Phlebotomus papatasi) CRISPR के लिए भ्रूण माइक्रोइंजेक्शन/

Article DOI: 10.3791/61924-v • 05:44 min • November 17th, 2020
November 17th, 2020 •
1, 1, 1, 1, 2, 2
1Laboratory of Parasitic Diseases, National Institute of Allergy and Infectious Diseases, National Institutes of Health, 2University of Maryland Insect Transformation Facility, The Institute for Bioscience and Biotechnology Research

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इस प्रोटोकॉल में रेत मक्खियों में CRISPR/Cas9 लक्षित म्यूटागेनेसिस के कदमों का विवरण दिया गया है: भ्रूण संग्रह, इंजेक्शन, कीट पालन, और पहचान के साथ-साथ ब्याज के उत्परिवर्तनों का चयन ।

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