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In vitro Synthesis of Native, Fibrous Long Spacing and Segmental Long Spacing Collagen
JoVE Journal
Bioengineering
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JoVE Journal Bioengineering
In vitro Synthesis of Native, Fibrous Long Spacing and Segmental Long Spacing Collagen

In vitro Synthesis of Native, Fibrous Long Spacing and Segmental Long Spacing Collagen

DOI:
10.3791/4417-v

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07:54 min

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September 20, 2012

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, , , ,
1Department of Chemistry,University of Toronto, 2Institute for Optical Sciences,University of Toronto

Chapters

  • 00:05Title
  • 01:06Preparation of Native Collagen Fibrils
  • 01:45Preparation of Fibrous Long Spacing Collagen
  • 02:35Preparation of Segmental Long Spacing Collagen
  • 03:19Atomic Force Microscopy Characterization of Collagen Fibrils
  • 05:10Results: AFM Verification of Collagen Fibril Type
  • 07:15Conclusion

Summary

Automatic Translation

Automatic Translation

Simple and reproducible procedures are described for making three structurally distinct collagen assemblies from a common commercially available Type I collagen monomer. Native type, fibrous long spacing or segmental long spacing collagen can be constructed by varying the conditions to which the 300 nm long and 1.4 nm diameter monomer building block is exposed.

Tags

In Vitro SynthesisNative CollagenFibrous Long Spacing CollagenSegmental Long Spacing CollagenExtracellular MatrixStructural ScaffoldingMechanical StrengthCollagen FibrilsBanding PeriodicityType I Collagen MonomersHierarchical AssemblyLength ScalesMicronsCommercially Available Collagen MonomerProtocolsMixtures Of StructuresUnbanded FibrilsImagingAtomic Force Microscope

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