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JoVE Journal
Bioengineering

This content is Open Access.

In vitro Synthesis of Native, Fibrous Long Spacing and Segmental Long Spacing Collagen
 

In vitro Synthesis of Native, Fibrous Long Spacing and Segmental Long Spacing Collagen

Article DOI: 10.3791/4417
September 20th, 2012

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Summary September 20th, 2012

Simple and reproducible procedures are described for making three structurally distinct collagen assemblies from a common commercially available Type I collagen monomer. Native type, fibrous long spacing or segmental long spacing collagen can be constructed by varying the conditions to which the 300 nm long and 1.4 nm diameter monomer building block is exposed.

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