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Journal / Biology / 전체 염색체, 텔로미어 및 유전자좌를 밝히기 위한 DNA Halo…
JoVE Journal
Biology

This content is Open Access.

JoVE Journal Biology
전체 염색체, 텔로미어 및 유전자좌를 밝히기 위한 DNA Halo Preparations의 형광 In Situ 혼성화
Identification of a Murine Erythroblast Subpopulation Enriched in Enucleating Events by Multi-spectral Imaging Flow Cytometry
Journal (Biology)
Identification of a Murine Erythroblast Subpopulation Enriched in Enucleating Events by Multi-spectral Imaging Flow Cytometry
Whole Mount in Situ Hybridization of E8.5 to E11.5 Mouse Embryos
Journal (Biology)
Whole Mount in Situ Hybridization of E8.5 to E11.5 Mouse Embryos
 
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전체 염색체, 텔로미어 및 유전자좌를 밝히기 위한 DNA Halo Preparations의 형광 In Situ 혼성화

Article DOI: 10.3791/62017-v • 09:07 min • March 4th, 2021
March 4th, 2021 •
1, 2, 1, 2
1Laboratory of Nuclear and Genomic Health, Centre for Genome Engineering and Maintenance, Division of Biosciences, Department of Life Sciences, College of Health, Medicine and Life Sciences, Brunel University London, 2Biosciences, Department of Clinical, Pharmaceutical and Biological Science, School of Life and Medical Sciences, University of Hertfordshire

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DNA 후광 제제와 형광 in situ 하이브리드화를 결합하면 뉴클레오스켈레톤과의 게놈 상호작용에 대한 고분해능 분석이 가능합니다. 부착된 게놈은 추출된 잔류 핵 내에 위치한 혼성화된 형광 신호를 유도하는 반면, 부착되지 않은 게놈은 잔류 핵을 둘러싸고 있는 DNA의 후광에 있습니다.

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이번 달 JoVE DNA 후광 준비 핵 매트릭스 뉴클레오스켈레톤 게놈 조직 DNA 루프 염색체 영역 확장된 염색질 섬유 매트릭스 부착 영역 텔로미어
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