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Journal / Biology / Fluorescens in situ hybridisering på DNA…
JoVE Journal
Biology

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JoVE Journal Biology
Fluorescens in situ hybridisering på DNA Halo-preparater for å avsløre hele kromosomer, telomerer og genloci
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Fluorescens in situ hybridisering på DNA Halo-preparater for å avsløre hele kromosomer, telomerer og genloci

Article DOI: 10.3791/62017-v • 09:07 min • March 4th, 2021
March 4th, 2021 •
1, 2, 1, 2
1Laboratory of Nuclear and Genomic Health, Centre for Genome Engineering and Maintenance, Division of Biosciences, Department of Life Sciences, College of Health, Medicine and Life Sciences, Brunel University London, 2Biosciences, Department of Clinical, Pharmaceutical and Biological Science, School of Life and Medical Sciences, University of Hertfordshire

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Kombinering av DNA-halopreparater med fluorescens in situ-hybridisering muliggjør høyoppløselig analyse av genomiske interaksjoner med nukleoskeletonet. Vedlagt genom fører til hybridiserte fluorescerende signaler lokalisert i de gjenværende ekstraherte kjernene, mens ikke-festet genom er i haloen av DNA som omgir de resterende kjernene.

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Denne måneden i JoVE utgave 169 DNA halo forberedelse kjernefysisk matrise nukleoskeleton genom organisasjon DNA-sløyfer kromosomterritorier utvidede kromatinfibre matrise vedlegg regioner telomerer
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