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JoVE Journal Behavior
Embryo Microinjection och Knockout Mutant Identification av CRISPR/Cas9 Genom-Redigerad Helicoverpa Armigera (Hübner)
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Embryo Microinjection och Knockout Mutant Identification av CRISPR/Cas9 Genom-Redigerad Helicoverpa Armigera (Hübner)

Article DOI: 10.3791/62068-v • 06:37 min • July 1st, 2021
July 1st, 2021 •
1,2, 2, 1, 1, 1, 2,3
1School of Chemical and Environmental Engineering, China University of Mining and Technology, 2State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, 3Guangdong Laboratory of Lingnan Modern Agriculture, Shenzhen; Agricultural Genomics Institute at Shenzhen, Chinese Academy of Agricultural Sciences

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Presenteras här är ett protokoll av Helicoverpa armigera (Hübner) embryo microinjection och knockout mutant identifiering skapad av CRISPR/Cas9 genomredigering. Mutanta insekter möjliggör ytterligare forskning om genfunktion och interaktion mellan olika gener in vivo.

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Beteende nummer 173 Helicoverpa armigera embryomikroinutskjutning gen knockouts CRISPR Cas9
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