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Biochemistry
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使用扩张显微镜对波多细胞蛋白尼腓林、阿克汀和波多辛的成像
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Biochemistry
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JoVE Journal
Biochemistry
Imaging of Podocytic Proteins Nephrin, Actin, and Podocin with Expansion Microscopy
Please note that all translations are automatically generated.
Click here for the English version.
使用扩张显微镜对波多细胞蛋白尼腓林、阿克汀和波多辛的成像
DOI:
10.3791/62079-v
•
06:18 min
•
April 23, 2021
•
Eva Königshausen*
1
,
Christina Theresa Schmitz*
1
,
Lars Christian Rump
,
Lorenz Sellin
1
Department of Nephrology, Medical Faculty
,
Heinrich-Heine-University
Chapters
00:05
Introduction
00:27
Preparation for Expansion Microscopy
01:24
Anchoring and Polymerization (gelation)
02:54
Homogenization (digestion)
04:14
Expansion and Imaging
05:04
Results: Podocin Co-localizes with Nephrin and Actin
05:46
Conclusion
Summary
Automatic Translation
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Automatic Translation
所介绍的方法使荧光标记的细胞蛋白具有扩展显微镜的可视化,从而在传统显微镜上实现 70 nm 的分辨率。
Tags
Expansion Microscopy
Podocyte
Nephrin
Actin
Podocin
Gelation Chamber
Anchoring Treatment
Phalloidin
Sodium Acrylate
Monomer Solution
APS
Gel Polymerization
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