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Podosit Proteinleri Nephrin, Actin ve Podocin'in Genleşme Mikroskopisi ile Görüntülenmesi
JoVE Journal
Biochemistry
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JoVE Journal Biochemistry
Imaging of Podocytic Proteins Nephrin, Actin, and Podocin with Expansion Microscopy
DOI:

06:18 min

April 23, 2021

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Chapters

  • 00:05Introduction
  • 00:27Preparation for Expansion Microscopy
  • 01:24Anchoring and Polymerization (gelation)
  • 02:54Homogenization (digestion)
  • 04:14Expansion and Imaging
  • 05:04Results: Podocin Co-localizes with Nephrin and Actin
  • 05:46Conclusion

Summary

Automatic Translation

Sunulan yöntem, floresan etiketli hücresel proteinlerin, geleneksel bir mikroskopta 70 nm çözünürlüğe yol açan genleşme mikroskopisi ile görselleştirilmesini sağlar.

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