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JoVE Journal Neuroscience
En pladebaseret analyse til måling af endogen monoaminfrigivelse i akutte hjerneskiver
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En pladebaseret analyse til måling af endogen monoaminfrigivelse i akutte hjerneskiver

Article DOI: 10.3791/62127-v • 07:56 min • August 11th, 2021
August 11th, 2021 •
*1, *2, 3, 2
1Department of Medicine, School of Medicine, Universidad de Atacama, 2Department of Molecular, Cellular, and Biomedical Sciences, City University of New York School of Medicine at City College, 3Department of Pharmacology and Therapeutics, University of Florida College of Medicine
* These authors contributed equally

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Denne metode introducerer en simpel teknik til påvisning af endogene monoamin frigivelse ved hjælp af akutte hjerne skiver. Opsætningen bruger en 48-brønd plade, der indeholder en vævsholder til monoamin frigivelse. Frigivet monoamin analyseres af HPLC kombineret med elektrokemisk detektion. Derudover, denne teknik giver en screening metode til opdagelse af lægemidler.

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Neurovidenskab monoamin frigivelse akut hjerne skiver monoaminer dopamin noradrenalin serotonin neurotransmission
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