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一种基于板的测定法,用于测量急性脑切片中内源性单胺释放
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A Plate-Based Assay for the Measurement of Endogenous Monoamine Release in Acute Brain Slices
Please note that all translations are automatically generated.
Click here for the English version.
一种基于板的测定法,用于测量急性脑切片中内源性单胺释放
DOI:
10.3791/62127-v
•
07:56 min
•
August 11, 2021
•
Jose A. Pino*
1
,
Nora Awadallah*
2
,
Alessandra M. Norris
,
Gonzalo E. Torres
1
Department of Medicine, School of Medicine
,
Universidad de Atacama
,
2
Department of Molecular, Cellular, and Biomedical Sciences
,
City University of New York School of Medicine at City College
,
3
Department of Pharmacology and Therapeutics
,
University of Florida College of Medicine
Chapters
00:04
Introduction
00:44
Acute Rat Brain Slice Preparation
02:01
Ex Vivo Endogenous Monoamine Release Induction
04:42
HPLC Analysis
05:29
Results: Representative Endogenous Monoamine Release in Acute Brain Slices
07:21
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
该方法引入了一种使用急性脑切片检测内源性单胺释放的简单技术。该装置使用含有组织支架的48孔板进行单胺释放。释放的单胺通过HPLC结合电化学检测进行分析。此外,该技术还为药物发现提供了一种筛选方法。
Tags
Endogenous Monoamine Release
Brain Slices
Plate-based Assay
Dorsal Striatum
Hippocampus
Efflux Buffer
Pharmacological Conditions
Vesicular Release
Transporter-mediated Release
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