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创伤性脑损伤后评估成年大鼠空间工作记忆的指标测试
Chapters
Summary May 7th, 2021
Please note that all translations are automatically generated.
Click here for the English version.
创伤性脑损伤 （TBI） 通常与记忆障碍相关。在此，我们提出一个协议，通过公制任务评估 TBI 之后的空间工作内存。公制测试是研究 TBI 后空间工作记忆障碍的有用工具。
Transcript
创伤性脑损伤通常与记忆障碍有关。在这里，我们提出了一个协议，通过一个指标任务来评估创伤性脑损伤后的空间工作记忆。公制任务的主要优点包括动态观测的可能性、低成本的可重复性、相对易于实施和低应力环境。
为了评估寄生液体打击乐损伤后的神经严重程度评分，将成年斯普拉格道利大鼠置于直径50厘米的圆的中心，三次并监测其退出圆的能力。要测试右反射的丧失，将动物放在手掌上，如果老鼠能够纠正自己，就给一个分数。要测试六肢瘫痪，评估大鼠抵抗强迫定位的能力。
要测试后肢的反射弯曲，将动物的尾巴抬起来。为了测试直走的能力，把老鼠放在地板上。要测试针刺反射，执行轻，触觉刺激观察耳朵缩回。
要测试角膜反射，请轻轻将软棒涂在眼睛上，并测量眨眼反应。要测试惊人的反射，将一支笔拖过铁丝网笼顶部，记录动物的反应。为了分级在假肢中寻找行为的损失，将大鼠置于一个新的环境中，并观察大鼠是移动其胡须、嗅觉还是奔跑。
然后测试肢体反射，以放置在左右前肢，然后是左后肢和右后肢。为了测试平衡，通过将鼠子放置在1.5厘米宽的光束上执行光束平衡任务，其时间为20、40和大于第二节。然后使用三个不同宽度的光束进行光束行走测试。
在执行公制任务之前，将一个直径为 200 厘米、厚 1 厘米的黑色圆形平台放置在 80 厘米高的桌子上，并将两个物体放置在平台中心，相距 68 厘米。在距离地面 290 厘米的高度安装带有所需计算机软件的摄像机，用于捕获、保存和处理数据。在分析前一天，将大鼠放在平台上10分钟，使动物适应新的环境。
在任务当天，将鼠放在平台的末端，与物体等距15分钟的习惯期，并开始记录。在实验习惯期结束时，将老鼠转移到单独的笼子里五分钟，用百分之五到百分之十的酒精清洁平台。将物体之间的距离缩小到34厘米，然后将老鼠放在平台上5分钟，同时记录其探索活动。
再次清洁平台，然后继续下一个动物。在分析视频文件之前，插入软件硬件密钥，然后启动视频跟踪软件并打开预设模板。在主视场验证、复制和重命名竞技场试用控制和检测设置后，右键单击鼠标以选择抓取背景。
在浏览菜单中选择视频文件的位置作为背景图像。捕获图像并标记被调查的区域和区域以校准图像以进行分析。对试用控制和检测设置执行相同的设置。
下载视频文件列表以供从试用列表中分析后，添加视频并指示具有所需设置的位置。然后选择获取并开始试用，并将所有数据导出到电子表格中。对照组没有显示任何神经缺陷，在假伤48小时后。
TBI之后，雄性及雌性受伤动物的神经缺陷显著增加。在指标任务期间，雄性 TBI 大鼠的对象探索时间明显短于雄性假操作鼠。雌性TBI和雌性假操作鼠也观察到同样的模式。
按照公制任务，我们可以执行其他记忆评估方法，如巴恩斯迷宫和拓扑和水迷宫任务。该指标任务还允许进一步研究神经损伤的可比模型中的记忆损伤，如弥漫性轴心损伤和中风模型。
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