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Investigations on Alterations of Hippocampal Circuit Function Following Mild Traumatic Brain Injury
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JoVE Journal Neuroscience
Investigations on Alterations of Hippocampal Circuit Function Following Mild Traumatic Brain Injury

Investigations on Alterations of Hippocampal Circuit Function Following Mild Traumatic Brain Injury

DOI:
10.3791/4411-v

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10:59 min

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November 19, 2012

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, , , , ,
1Division of Neurology,Children’s Hospital of Philadelphia, 2Neuroscience Graduate Group,Perelman School of Medicine at the University of Pennsylvania, 3Department of Pediatrics,Perelman School of Medicine at the University of Pennsylvania

Chapters

  • 00:05Title
  • 02:08Lateral Fluid Percussion Injury
  • 03:04Behavioral Analysis- Conditioned Fear Response
  • 03:42Acute Hippocampal Slice Preparation
  • 04:41Extracellular Field Potential Recording and Visualized Patch-clamp Recording
  • 06:43Voltage Sensitive Dye Imaging
  • 08:39Results: Alterations of Hippocampal Circuit Function Following Mild Traumatic Brain Injury
  • 10:33Conclusion

Summary

Automatic Translation

Automatic Translation

A multi-faceted approach to investigating functional changes to hippocampal circuitry is explained. Electrophysiological techniques are described along with the injury protocol, behavioral testing and regional dissection method. The combination of these techniques can be applied in similar fashion for other brain regions and scientific questions.

Tags

InvestigationsAlterationsHippocampal Circuit FunctionMild Traumatic Brain InjuryTBINeurological ImpairmentsMemory DeficitsSeizure ThresholdHippocampal DysfunctionLateral Fluid Percussion InjuryAnimal ModelNeuronal Cell LossGliosisIonic PerturbationCombinatorial MethodEx Vivo Physiological TechniquesAnimal Behavior AnalysisBiochemical AnalysisCognitive DisabilityExtracellular Field Potential RecordingWhole-cell Patch-clampingVoltage Sensitive Dye RecordingRegionally Dissecting Subregions Of The HippocampusNeurochemical AnalysisMetabolic Analysis

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