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Journal / Cancer Research / 멤브레인 결합-IL-21-변형 B 세포주를 이용한 자연살해(NK) 및 CAR-NK 세포…
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Cancer Research

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JoVE Journal Cancer Research
멤브레인 결합-IL-21-변형 B 세포주를 이용한 자연살해(NK) 및 CAR-NK 세포 확장 방법
Droplet Digital TRAP (ddTRAP): Adaptation of the Telomere Repeat Amplification Protocol to Droplet Digital Polymerase Chain Reaction
Journal (Cancer Research)
Droplet Digital TRAP (ddTRAP): Adaptation of the Telomere Repeat Amplification Protocol to Droplet Digital Polymerase Chain Reaction
Study of Viral Vectors in a Three-dimensional Liver Model Repopulated with the Human Hepatocellular Carcinoma Cell Line HepG2
Journal (Cancer Research)
Study of Viral Vectors in a Three-dimensional Liver Model Repopulated with the Human Hepatocellular Carcinoma Cell Line HepG2
 
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멤브레인 결합-IL-21-변형 B 세포주를 이용한 자연살해(NK) 및 CAR-NK 세포 확장 방법

Article DOI: 10.3791/62336-v • 11:02 min • February 8th, 2022
February 8th, 2022 •
1,2, 1, 1, 1,3
1Department of Pathology, Immunology and Laboratory Medicine, Rutgers-New Jersey Medical School, 2Department of Microbiology, Biochemistry & Molecular Genetics, Public Health Research Institute Center, New Jersey Medical School, Rutgers University, 3Center for Immunity and Inflammation, New Jersey Medical School, Rutgers-The State University of New Jersey

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여기에서는 말초혈액자연살해(PBNK), 간 조직으로부터 NK세포, 말초혈액단핵세포(PBMC) 또는 제대혈(CB)에서 유래한 키메라 항원 수용체(CAR)-NK세포를 확장시키는 방법을 제시한다. 이 프로토콜은 확장된 NK 세포의 최적화된 순도 외에도 221-mIL-21 영양세포를 사용하는 NK 및 CAR-NK 세포의 확장을 보여줍니다.

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암 연구 180 호
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