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Journal / Cancer Research / Metodo di espansione cellulare Natural Killer…
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JoVE Journal Cancer Research
Metodo di espansione cellulare Natural Killer (NK) e CAR-NK utilizzando la linea cellulare B modificata Membrane Bound-IL-21-Modified
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Metodo di espansione cellulare Natural Killer (NK) e CAR-NK utilizzando la linea cellulare B modificata Membrane Bound-IL-21-Modified

Article DOI: 10.3791/62336-v • 11:02 min • February 8th, 2022
February 8th, 2022 •
1,2, 1, 1, 1,3
1Department of Pathology, Immunology and Laboratory Medicine, Rutgers-New Jersey Medical School, 2Department of Microbiology, Biochemistry & Molecular Genetics, Public Health Research Institute Center, New Jersey Medical School, Rutgers University, 3Center for Immunity and Inflammation, New Jersey Medical School, Rutgers-The State University of New Jersey

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Qui, presentiamo un metodo per espandere il natural killer del sangue periferico (PBNK), le cellule NK dai tessuti epatici e le cellule del recettore dell'antigene chimerico (CAR)-NK derivate da cellule mononucleate del sangue periferico (PBMC) o sangue del cordone ombelicale (CB). Questo protocollo dimostra l'espansione delle celle NK e CAR-NK utilizzando celle di alimentazione 221-mIL-21 oltre alla purezza ottimizzata delle celle NK espanse.

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