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JoVE Journal Cancer Research
Natural Killer (NK) och CAR-NK cellexpansionsmetod med membranbunden IL-21-modifierad B-cellinje
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Natural Killer (NK) och CAR-NK cellexpansionsmetod med membranbunden IL-21-modifierad B-cellinje

Article DOI: 10.3791/62336-v • 11:02 min • February 8th, 2022
February 8th, 2022 •
1,2, 1, 1, 1,3
1Department of Pathology, Immunology and Laboratory Medicine, Rutgers-New Jersey Medical School, 2Department of Microbiology, Biochemistry & Molecular Genetics, Public Health Research Institute Center, New Jersey Medical School, Rutgers University, 3Center for Immunity and Inflammation, New Jersey Medical School, Rutgers-The State University of New Jersey

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Här presenterar vi en metod för att expandera perifert blod naturligt dödare (PBNK), NK-celler från levervävnader och chimär antigenreceptor (CAR) -NK-celler härledda från mononukleära celler i perifert blod (PBMC) eller navelsträngsblod (CB). Detta protokoll visar expansionen av NK- och CAR-NK-celler med användning av 221-mIL-21-matarceller utöver den optimerade renheten hos expanderade NK-celler.

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Cancerforskning nummer 180
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