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穆林肺瓣膜相关成像的手术和样品处理
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Engineering
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JoVE Journal Engineering
Surgery and Sample Processing for Correlative Imaging of the Murine Pulmonary Valve
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穆林肺瓣膜相关成像的手术和样品处理

DOI:
10.3791/62581-v

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07:34 min

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August 05, 2021

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, , , , , , ,
1Center for Electron Microscopy and Analysis, The Department of Materials Science and Engineering,Ohio State University, 2Center for Regenerative Medicine,Nationwide Children’s Hospital, 3Thermo Fisher Scientific

Chapters

  • 00:04Introduction
  • 00:50Pulmonary Valve Excision
  • 02:00Pressure Fixation of Pulmonary Valve
  • 03:19En Bloc Sample Staining and Embedment
  • 05:48Results: Correlative Imaging of the Murine Pulmonary Valve
  • 07:10Conclusion

Summary

Automatic Translation

Automatic Translation

在这里，我们描述了一个相关的工作流程的切除，加压，固定和成像的粘液肺瓣，以确定总构象和局部细胞外矩阵结构。

Tags

Murine Pulmonary ValveCorrelative ImagingStructure-functionControlled FixationHigh-resolution ImagingMouse DissectionThoracotomyPulmonary Artery PressurizationPrimary Fixative Solution

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