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Kirurgi og prøvebehandling for korrelerativ billeddannelse af Murine lungeventilen
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Engineering
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JoVE Journal Engineering
Surgery and Sample Processing for Correlative Imaging of the Murine Pulmonary Valve

Kirurgi og prøvebehandling for korrelerativ billeddannelse af Murine lungeventilen

DOI:
10.3791/62581-v

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07:34 min

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August 05, 2021

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, , , , , , ,
1Center for Electron Microscopy and Analysis, The Department of Materials Science and Engineering,Ohio State University, 2Center for Regenerative Medicine,Nationwide Children’s Hospital, 3Thermo Fisher Scientific

Chapters

  • 00:04Introduction
  • 00:50Pulmonary Valve Excision
  • 02:00Pressure Fixation of Pulmonary Valve
  • 03:19En Bloc Sample Staining and Embedment
  • 05:48Results: Correlative Imaging of the Murine Pulmonary Valve
  • 07:10Conclusion

Summary

Automatic Translation

Automatic Translation

Her beskriver vi en korrelativ arbejdsgang for excision, tryk, fiksering og billeddannelse af murinepulmonalventilen for at bestemme bruttokonformationen og lokale ekstracellulære matrixstrukturer.

Tags

Murine Pulmonary ValveCorrelative ImagingStructure-functionControlled FixationHigh-resolution ImagingMouse DissectionThoracotomyPulmonary Artery PressurizationPrimary Fixative Solution

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