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Preparação de membrana plasmática em pequena escala para a análise de cânbicas cânbicas cdr1-mGFPHis
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Preparação de membrana plasmática em pequena escala para a análise de cânbicas cânbicas cdr1-mGFPHis

Article DOI: 10.3791/62592-v • 09:44 min • June 13th, 2021
June 13th, 2021 •
*1, *1, 1, 1,2, 3, 1
1Sir John Walsh Research Institute, Faculty of Dentistry, University of Otago, 2Department of Microbiology, Faculty of Medicine, Chulalongkorn University, 3School of Biological Sciences, University of Auckland
* These authors contributed equally

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Este artigo apresenta um protocolo de isolamento de membrana plasmática de pequena escala para a caracterização da proteína Cdr1 (cassete de ligação ATP) da Candida ABC (fita adesão a ATP), superexpressa em Saccharomyces cerevisiae. Uma mGFPC terminal de protease com um linker de 16 resíduos entre o Cdr1 e a tag foi projetada para facilitar a purificação e o detergente-triagem do Cdr1.

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Biologia Edição 172
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